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fermentasRNA酶抑制劑RiboLock RNase Inhibitor

  • 更新時(shí)間:  2023-07-24
  • 產(chǎn)品型號(hào):  EO0381
  • 簡(jiǎn)單描述
  • fermentasRNA酶抑制劑RiboLock RNase Inhibitor
    應(yīng)用范圍廣,適合多種使用條件。
    保護(hù)RNA,使其免受降解。Z高有效溫度達(dá)55°C
    規(guī)格單位:2500U 4*2500U
詳細(xì)介紹

fermentasRNA酶抑制劑RiboLock RNase Inhibitor

 Description

Thermo Scientific RiboLock RNase Inhibitor inhibits the activity of RNases A (see Figure 1 in Supporting Data), B, and C by binding them in a noncompetitive mode at a 1:1 ratio. It does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H.

Highlights

  • Performs under a wide range of reaction conditions
  • Protects RNA from degradation at temperatures up to 55°C (see Figure 2 in Supporting Data)

Note

DTT provided in the Storage Buffer ensures stability during long term storage, but is not necessary for inhibitor activity.

 

Specifications

CategoryName 
Concentration40 U/µL
Definition of Activity Unit
  • One unit of the RiboLock RNase Inhibitor inhibits the activity of 5 ng RNase A by 50%.
  • Inhibitor activity is assayed in the following mixture: 100 mM Tris-HCl (pH 7.5), 1.2 mM EDTA, 0.1 mg/mL BSA, 100 ng/mL RNase A, 0.1 mg/mL [3H]-RNA, 50 mg/mL yeast RNA, 8 mM DTT.
HazardousNo
Hazardous:No
InactivationInactivated by heating at 75°C for 10 min. Residual activity detectable after 10 min heating at 70°C.
InhibitionInhibitors: common denaturants (SDS, urea and all oxidizing reagents (p-chloromercuribenzoate, dissolved oxygen, ions in their higher oxidation states) strongly inhibit RiboLock RNase Inhibitor and release the RNase bound.
Molecular Weight49.6 kDa monomer
Quality Control
  • The absence of ribonucleases, endo-, exodeoxyribonucleases and phosphatases confirmed by appropriate quality tests.
  • Functionally tested in RNA and cDNA synthesis.
Shelf Life: 
Shipping Condition: 
Shipping Information 
SourceE.coli cells with a cloned gene encoding mammalian ribonuclease inhibitor.
SpecificationName 
SpecificationValue 
Storage BufferThe enzyme is supplied in 20 mM HEPES-NaOH (pH 7.5), 50 mM NaCl, 8 mM DTT, 0.03% v/v) ELUGENT Detergent and 50% (v/v) glycerol.
Storage Condition-20 C

Storage Condition:

 

 

fermentasRNA酶抑制劑RiboLock RNase Inhibitor *

*明細(xì):

DreamTaq Green PCR Master Mix (2×)

K1081

200rxns

500

300

K1082

1000rxns

2,000

1200

DreamTaq PCR Master Mix (2×)

K1071

200rxns

500

300

K1072

1000rxns

2,000

1200

PCR master Mix (2×)

K0171

200rxns

450

276

K0172

1000rxns

1,800

1080

Long PCR Enzyme Mix

K0181

100U

250

150

K0182

500U

1,000

600

DreamTaq DNA Polymerase

EP0701

200U

150

90

EP0702

500U

200

120

EP0703

5x500U

900

540

EP0704

20x500U

3,200

1920

DreamTaq Green DNA Polymerase

EP0711

200U

150

90

EP0712

500U

200

120

EP0713

5x500U

1,080

648

EP0714

20x500U

3,200

1920

TurboFect in vitro Transfection Reagent

R0531

1ml

2,000

1300

TurboFect siRNA Transfection Reagent

R1401

0.5ml

1,600

1040

TurboFect Protein Transfection Reagent

R1411

125ul

2,140

1391

TurboFect in vivo Transfection Reagent

R0541

120ul

3,000

1950

 


“贈(zèng)送送到Hi”
 

品名

貨號(hào)

包裝

目錄價(jià)¥

贈(zèng)送說(shuō)明

RevertAid First Strand cDNA Synthesis Kit

K1622

100rxns

1,600

買(mǎi)2個(gè),贈(zèng)1個(gè)K1081(DreamTaq Green PCR Master Mix),價(jià)值¥500

Maxima First Strand cDNA Synthesis Kit

K1641

50rxns

2,500

同時(shí)購(gòu)買(mǎi),贈(zèng)2個(gè)K1081(DreamTaq Green PCR Master Mix),價(jià)值¥1000

GeneJET RNA Purification Kit

K0731

50preps

1,900

Maxima First Strand cDNA Synthesis Kit

K1642

200rxns

7,500

同時(shí)購(gòu)買(mǎi),贈(zèng)2個(gè)K1082(DreamTaq Green PCR Master Mix),價(jià)值¥4000

GeneJET RNA Purification Kit

K0732

250preps

7,600

RevertAid Premium First Strand cDNA Synthesis Kit

K1651

50rxns

970

同時(shí)購(gòu)買(mǎi),贈(zèng)2個(gè)K1081(DreamTaq Green PCR Master Mix),價(jià)值¥1000

GeneJET RNA Purification Kit

K0731

50preps

1,900

RevertAid Premium First Strand cDNA Synthesis Kit

K1652

200rxns

3,880

同時(shí)購(gòu)買(mǎi),贈(zèng)2個(gè)K1082(DreamTaq Green PCR Master Mix),價(jià)值¥4000

GeneJET RNA Purification Kit

K0732

250preps

7,600

Maxima Reverse Transcriptase

EP0741

2000U

450

買(mǎi)1個(gè),贈(zèng)1個(gè)EO0381(RiboLock RNase Inhibitor),價(jià)值¥200

EP0742

10000U

1450

買(mǎi)1個(gè),贈(zèng)2個(gè)EO0381(RiboLock RNase Inhibitor),價(jià)值¥400

RevertAid Premium Reverse Transcriptase

EP0731

2000U

420

買(mǎi)1個(gè),贈(zèng)1個(gè)EO0381(RiboLock RNase Inhibitor),價(jià)值¥200

EP0732

10000U

1400

買(mǎi)1個(gè),贈(zèng)2個(gè)EO0381(RiboLock RNase Inhibitor),價(jià)值¥400

 

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